Papillary lesion histology
Tumor angiogenesis in gastric cancer Daniela Lazăr, M.
Atlas of Breast Pathology - wishstudio.ro
Raica, I. Sporea, Sorina Tăban, A.
Goldiş, Marioara Cornianu Growth of solid tumors, including gastric cancer, and the formation of metastasis depend on the induction of new blood vessels; in tumors, angiogenesis is uncontrolled and immature. This is a complex process, depending on a great variety of angiogenic factors, one of the most important being the vascular endothelial growth factor. In order to suppress the tumor development and the occurrence of metastasis, clinical trials have been developed with angiogenesis inhibitors, many of them with encouraging results.
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Further research is needed in regard to the idea of combined antiangiogenic therapy with conventional chemotherapy, or even immune or genetic therapies, in order to increase treatment efficiency and to suppress side effects. Vollmer, J.
Galle, D. Lang, S. Loeschke, H. Schultz, T.
Papillary urothelial tumor of low malignant potential Mircea O. Mariusz Z. Kevin R. Stephen P. Richard J.
Goldmann Fixation of tissues with formalin results in well-preserved morphology but to a high degree leads to degradation of nucleic acids, which substantially constricts the spectrum of applicable molecular techniques. The novel HOPE-fixative with subsequent paraffin embedding, as an alternative to formalin, has been shown to result in a morphological preservation comparable to formalin-fixed, paraffin-embedded specimens.
Due to a similar workflow like in formalin-fixation and paraffin embedding, the HOPE technique can be successfully established within any pathological institute.
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Moreover, we described procedures which permit successful application of all common molecular techniques such as in situ hybridization targeting either DNA or RNA, immunohistochemistry without antigen retrieval and for formalin-refractory antigens, PCR, RT-PCR, Western blot, Northern blot, and transcription microarrays to HOPE-fixed, paraffin-embedded tissues. Furthermore, HOPE-fixed tissues can be used for the construction of tissue microarrays for enhanced high-throughput analyses on the molecular level.
Using the HOPE technique as its crucial methodological base, ex vivo model systems could be established, e.
In addition to tissues, cell-culture preparations have been prepared utilizing the HOPE technique, which were then successfully applied to in situ hybridization targeting mRNA or immunocytochemistry with excellent preservation of morphological details. Taken together, the HOPE technique to date represents an alternative fixation that is, in contrary to other procedures, scientifically broadly analyzed.
Therefore, new possibilities are opened up especially within the rapidly growing field of molecular pathology.
Atlas of Breast Pathology Papillary breast lesion with atypia. Carcinom folicular vs adenomatos Therefore, it is a major challenge of both diagnosis and treatment.
Digitized pathology: theory and experiences in automated tissue-based virtual diagnosis Papillary lesion histology. Kayser, D. Radziszowski, P.
ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY
Bzdyl, R. Sommer, G. Kayser Aims.
To describe the theory and develop an automated virtual papillary lesion histology screening system. Theoretical considerations. Tissue-based diagnosis separates into a sampling procedure to allocate the slide area containing diagnostic information, and b evaluation of diagnosis from the selected area.
Nyquist's theorem broadly applied in acoustics, serves papillary lesion histology presetting the sampling accuracy.
Papillary breast lesion with atypia. Carcinom folicular vs adenomatos
Tissue-based diagnosis relies on two different information systems: a texture, and b object information. Texture information can be derived by recursive formulas without image segmentation.
Object information requires image segmentation and feature extraction. Both algorithms complete another to a 'self-learning' classification system. Non-overlapping compartments of the original virtual slide image are chosen at random with predefined error-rate Nyquist's theorem. The standardized image compartments are subject for texture and object analysis.